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      您的位置: 首頁>產品中心> Recombinant VHH>Alpaca Anti- Taq, Recombinant VHH
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      Alpaca Anti- Taq, Recombinant VHH

      Category: Recombinant VHH

      Alpaca and Llama heavy chain only antibodies provide a framework for engineering recombinant antibodies. The monomeric variable domain (VHH) of heavy chain only camelid IgG2 and IgG3 provides a 12-15 kDa domain which is easy to clone and produce at high levels in recombinant systems, is stable to heat and pH extremes, and generally, has good solubility.

      Alpaca Anti-Taq, Recombinant  VHH is a nanobody that binds Taq polymerase and inhibits its activity until reaction temperature is elevated. At that point, the Taq Antibody is denatured and releases its hold on Taq polymerase, allowing DNA synthesis to proceed. The binding of the Taq Antibody to Taq polymerase is the premise of hot-start PCR.By preventing Taq polymerase activity until the denaturation step of PCR is underway, nonspecific amplification due to mispriming and/or formation of primer dimers during PCR assembly is prevented. As a result, experimental background is significantly decreased, and reaction specificity is increased.

      Product overview Documents References FAQ

      Description

      Alpaca Anti-Taq, Recombinant  VHH, purified recombinant binding protein

      Affinity

      Dissociation constant KD of 0.1nM

      Applications

      Hot-start PCR

      Antibody performance

      Following incubation of Anti- Taq, Recombinant  VHH and Taq polymerase at 25°C for 10 min, inhibition of Taq polymerase activity was confirmed as greater than 99% (at 55°C for 10 min).

      Following heat treatment at 94°C for 30 seconds, Taq antibody-bound Taq polymerase was confirmed to recover almost 100% activity (at 55°C for 10 minutes).

      Benefits:

      Recombinant expresion  by E.coli: Eliminates batch-to-batch variations

      High binding affinity: Dissociation constants down to 0.1 nM


      Cat / Size / Price

      500U/¥260.0 2000U/¥1000.0

      Contact

      If you want to sell VHH on our platform, please contact us !

      Phone

      13551086942

      Email

      service@nb-biolab.com

      聯系

      企業郵箱:service@nb-biolab.com


      公司地址:成都市溫江區青啤大道319號


      上班時間:周一至周五


      工作時間:9:00-18:00


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      咨詢電話:400-166-9953


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