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      您的位置: 首頁>產品中心>Nano-secondary antibody>Alpaca Anti-Rabbit IgG, Recombinant VHH (HRP)
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      Alpaca Anti-Rabbit IgG, Recombinant VHH (HRP)

      Category: Nano-secondary antibody

      Alpaca and Llama heavy chain only antibodies provide a framework for engineering recombinant antibodies. The monomeric variable domain (VHH) of heavy chain only camelid IgG2 and IgG3 provides a 12-15 kDa domain which is easy to clone and produce at high levels in recombinant systems, is stable to heat and pH extremes, and generally, has good solubility. Alpaca Anti-Rabbit IgG,Recombinant VHH (HRP) is an anti-rabbit IgG specific secondary antibody. It consists of of a mixture of 3 anti-rabbit Nanobodies that bind to rabbit IgG FC with high affinity & specificity.

      Product overview Documents References FAQ
      • Summary

      Immunogen:Immunogen was Fc region of Recombinant Rabbit IgG

      Specificity:This Recombinant single-domain antibody is specific to Rabbit IgG FC region

      Isotype:VHH domain of alpaca IgG2a/2b

      Clonality:mixture of 3 clones

      Host:Alpaca pacous

      Purity:Affinity purified

      Affinity:VHH1=0.284nM VHH2=0.026nM VHH3<1pM

      Cross Reacivty:No Cross-reactivity to human ,macaque mouse, rat,sheep,Goat and guinea pig IgG

      Synonyms:Alpaca single domain antibody,VHH,Nanobody

      Conjugate:HRP


      • Applications/Dilutions

      Dilutions:Western Blot(WB) 1:100000 - 1:600000

      NB001H are monovalent and high affinity antibodies that don’t cluster. Hence, they enable one-step WB: the simultaneous incubation of primary and secondary antibodies. This optional method saves incubation time and washing steps.

      ELISA:1:5000-1:10000

      Immunohistochemistry(IHC):1:500 - 1:1000



      • Packaging, Storage & Formulations

      Storage:Store at -20℃

      Buffer:Phosphate Buffered Saline (PBS) containing 0.2%BSA,50% glycerol

      Preservative:0.1% Pro-Clean 400

      Concentration:1.0 mg/ml

      Purity:Recombinant Expression and Affinity purified

      This product is for research use only and is not approved for use in humans or in clinical diagnosis.


      資源 6.jpg


      Fig1: Western blot analysis of rabbit anti-GAPDH antibody (ET1601-4,1/2000) and rabbit anti-GST3 antibody (ER1802-63, 1/1000) with K562 Cell and A549 Cell lysates (All lanes proteins at 10 μg per lane). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBST for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Alpaca anti-rabbit IgG , recombinant VHH (HRP) (NB001H) was used for 1 hour at room temperature at 1:40,000 (Lane A, E), 1:80,000 (Lane B, F), 1:160,000 (Lane C, G) and 1:320,000 (Lane D, H).

      Exposure time: 30 seconds

      資源 8.jpg


      Fig2: Immunohistochemical analysis of paraffin-embedded human Colorectal cancer tissue using rabbit anti-Cytokeratin 18 antibody.

      The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1603-8, 1/200 dilution) for 30 minutes at room temperature. Alpaca anti-rabbit IgG, recombinant VHH (HRP) (NB001H, 1/200 dilution) was used for 30 min at room temperature. Tissues were counterstained with hematoxylin and mounted with DPX.

      The inset negative control image is secondary-only at 1/200 dilution.


      資源 9.jpg

      Fig3: Immunohistochemical analysis of paraffin-embedded human Kidney tissue using rabbit anti-CD13 antibody.

      The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1803-45, 1/200) for 30 minutes at room temperature. Alpaca anti-rabbit IgG Fc, recombinant VHH (HRP) (NB001H, 1/200 dilution) was used for 30 min at room temperature. Tissues were counterstained with hematoxylin and mounted with DPX.



      Contact

      If you want to sell VHH on our platform, please contact us !

      Phone

      13551086942

      Email

      service@nb-biolab.com

      聯系

      企業郵箱:service@nb-biolab.com


      公司地址:成都市溫江區青啤大道319號


      上班時間:周一至周五


      工作時間:9:00-18:00


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