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      您的位置: 首頁>產品中心>Nanoselector>GFP Nanoselector Agarose
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      GFP Nanoselector Agarose

      Category: Nanoselector

      GFP

      Product overview Documents References FAQ

      Introduction

      Green fluorescent proteins (GFPs) and variants thereof are widely used to study proteinlocalization and dynamics. For biochemical analysis including mass spectrometry and enzymeactivity measurements these GFP-fusion proteins and their interacting factors can be isolated fast and efficiently by immunoprecipitation using the GFP nanoselector Agarose .GFP nanoselector Agarose   utilizes small recombinant alpaca antibody fragments covalently coupled to the surface of Agarose beads.

      Specificity

      GFP Smartcapture? beads was tested on eGFP, GFP, tagGFP, YFP, CFP, Venus, Citrine, AcGFP.

      Affinity

      KD<1pM

      Bead properties

      Bead size: ~ 90 μm 

      Storage buffer: 20% EtOH

      Binding capacity

      High binding capacity,10 μL slurry bind about 25 μg of recombinant GFP

      Elution

      - SDS sample buffer
      - 0.2 M glycine pH 2.5
      Instead of elution, we recommend on-bead assays like on-bead digestion for MS analysis.

      Stability and Storage

      Shipped at ambient temperature. Upon receipt store at +4°C. Stable for 1 year. Do not freeze.

      Benefits

      • Effective pulldown of GFP-fusion proteins for consistent results

      • No heavy & light antibody chains, short incubation (5-30 min)

      • Extraordinary binding, also under harsh conditions

      • Very high affinity (KD<1 pM) to bind even low abundant protein


      GFP

      Contact

      If you want to sell VHH on our platform, please contact us !

      Phone

      13551086942

      Email

      service@nb-biolab.com

      聯系

      企業郵箱:service@nb-biolab.com


      公司地址:成都市溫江區青啤大道319號


      上班時間:周一至周五


      工作時間:9:00-18:00


      咨詢

      咨詢電話:400-166-9953


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