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      您的位置: 首頁>產品中心>Nanoselector>mNeogreen Nanoselector Agarose
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      mNeogreen Nanoselector Agarose

      Category: Nanoselector

      Product overview Documents References FAQ

      Introduction

      mNeonGreen is the brightest monomeric green or yellow fluorescent protein yet described to ourknowledge, performs exceptionally well as a fusion tag for traditional imaging as well as stochastic single molecule super-resolution imaging and is an excellent fluorescence resonance energy transfer(FRET) acceptor for the newest cyan fluorescent proteins. With the mNeogreen Nanoselector, specific for this non-GFP variant, yields of fusion proteins purification are high and free from heavy and light-chain contaminants, all in under 30 minutes. Save time, save effort and get cleaner results. The mNeogreen Nanoselector is the use of super-high affinity Alpaca antibody fragments called Nanobodies. mNeogreen Nanoselector may be used for immuno-precipitation, immuno-purification and immuno-pull down experiments with up to 10-fold better purity and yield than that of classic mouse monoclonal antibodies. Compatible with a variety of source materials, Nanoselector may be used with mammalian cells, tissues & organs, bacteria, yeast and even plants. These reagents allow your mNeonGreen-fusions to be perfect candidates for immune-precipitations, Co-IP, mass spectroscopy, and enzyme activity measurements.


      Specificity

      mNeogreen Nanoselector was tested on mNeonGreen 


      Affinity

      0.2nM


      Bead properties

      Bead size: ~ 90 μm 

      Storage buffer: 20% EtOH


      Binding capacity
      High binding capacity,10 μL slurry bind about 20 μg of recombinant mNeogreen


      Elution
      - SDS sample buffer
      - 0.2 M glycine pH 2.5
      Instead of elution, we recommend on-bead assays like on-bead digestion for MS analysis.


      Stability and Storage

      Shipped at ambient temperature. Upon receipt store at +4°C. Stable for 1 year. Do not freeze.


      Benefits

      • Clean immunoprecipitation of mNeonGreen-fusion proteins

      • No heavy & light chains in your downstream application

      • Strong binding even under harsh washing conditions

      • Short incubation time of about 30 minutes


      Contact

      If you want to sell VHH on our platform, please contact us !

      Phone

      13551086942

      Email

      service@nb-biolab.com

      聯系

      企業郵箱:service@nb-biolab.com


      公司地址:成都市溫江區青啤大道319號


      上班時間:周一至周五


      工作時間:9:00-18:00


      咨詢

      咨詢電話:400-166-9953


      官方微信

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