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      turboGFP Nanoselector Agarose

      Category: Nanoselector

      turboGFP

      Product overview Documents References FAQ

      Introduction

      The dimeric green fluorescent protein TurboGFP is derived from the green fluorescent protein CopGFP of the copepod Pontellina plumata (Shagin et al., 2004). It possesses bright green fluorescence with excitation maximum at 482 nm and emission maximum at 502 nm. TurboGFP is a fast maturating protein: its fluorescent signal is visible earlier than other green fluorescent proteins. TurboGFP shares only about 20% sequence identity with jellyfish GFP variants. Therefore, most anti-GFP antibodies including the GFP-Nanobody used in GFP-Trap do not bind to TurboGFP. TurboGFP is mainly intended for applications where fast appearance of bright fluorescence is crucial. It is specially recommended for cell and organelle labeling and tracking the promoter activity. Destabilized TurboGFP variant allows accurate analysis of rapid and/or transient events in gene regulation.


      Specificity

      TurboGFP and CopGFP from the copepod Pontellina plumata.
      The TurboGFP-Trap does not bind to jellyfish GFP and derivatives


      Affinity

      0.18nM


      Bead properties

      Bead size: ~ 90 μm 

      Storage buffer: 20% EtOH


      Binding capacity
      High binding capacity,10 μL slurry bind about 25 μg of recombinant TurboGFP


      Elution
      - SDS sample buffer
      - 0.2 M glycine pH 2.5
      Instead of elution, we recommend on-bead assays like on-bead digestion for MS analysis.


      Stability and Storage

      Shipped at ambient temperature. Upon receipt store at +4°C. Stable for 1 year. Do not freeze.


      Benefits

      • Effective pull-down of TurboGFP fusion proteins, low background

      • NO heavy & light chain contamination

      • High binding affinity

      • Harsh washing conditions

      turboGFP

      Contact

      If you want to sell VHH on our platform, please contact us !

      Phone

      13551086942

      Email

      service@nb-biolab.com

      聯系

      企業郵箱:service@nb-biolab.com


      公司地址:成都市溫江區青啤大道319號


      上班時間:周一至周五


      工作時間:9:00-18:00


      咨詢

      咨詢電話:400-166-9953


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