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      您的位置: 首頁>產品中心>Nanoselector>MBP Nanoselector Agarose
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      MBP Nanoselector Agarose

      Category: Nanoselector

      MBP

      Product overview Documents References FAQ

      Introduction

      MBP is used to increase the solubility of recombinant proteins expressed in E. coli. In these systems, the protein of interest is often expressed as a MBP-fusion protein, preventing aggregation of the protein of interest. The mechanism by which MBP increases solubility is not well understood. In addition, MBP can itself be used as an affinity tag for purification of recombinant proteins. The fusion protein binds to amylose columns while all other proteins flow through.MBP Nanoselector Agarose  is an affinity resin for immunoprecipitation of MBP fusion proteins. The MBP Nanoselector consists of an MBP Nanobody/ VHH coupled to agarose beads.


      Specificity

      Maltose binding protein (MBP), E. coli


      Affinity

      0.13nM


      Bead properties

      Bead size: ~ 90 μm 

      Storage buffer: 20% EtOH


      Binding capacity
      High binding capacity,10 μL slurry bind about 20 μg of recombinant MBP


      Elution
      - SDS sample buffer
      - 0.2 M glycine pH 2.5
      Instead of elution, we recommend on-bead assays like on-bead digestion for MS analysis.


      Stability and Storage

      Shipped at ambient temperature. Upon receipt store at +4°C. Stable for 1 year. Do not freeze.


      Benefits

      • Effective pull-down of MBP fusion proteins, low background

      • Significantly higher sensitivity than amylose resin and other affinity resins

      • NO heavy & light chain contamination

      • High binding affinity

      • Harsh washing conditions


      Contact

      If you want to sell VHH on our platform, please contact us !

      Phone

      13551086942

      Email

      service@nb-biolab.com

      聯系

      企業郵箱:service@nb-biolab.com


      公司地址:成都市溫江區青啤大道319號


      上班時間:周一至周五


      工作時間:9:00-18:00


      咨詢

      咨詢電話:400-166-9953


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