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      您的位置: 首頁>產品中心>Nanoselector>GST Nanoselector Agarose
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      GST Nanoselector Agarose

      Category: Nanoselector

      GST

      Product overview Documents References FAQ

      Introduction

      Glutathione S-transferases (GSTs), previously known as ligandins, comprise a family of eukaryotic and prokaryotic phase II metabolic isozymes best known for their ability to catalyze the conjugation of the reduced form of glutathione (GSH) to xenobiotic substrates for the purpose of detoxification. GST can be added to a protein of interest to purify it from solution in a process known as a pull-down assay. This is accomplished by inserting the GST DNA coding sequence next to that which codes for the protein of interest. Thus, after transcription and translation, the GST protein and the protein of interest will be expressed together as a fusion protein.GST Nanoselector Agarose  is a ready to use resin for immunoprecipitation/pulldown of GST fusion proteins.The GST-Trap consists of a GST Nanobody /VHH coupled to agarose beads.


      Specificity

      Glutathione S-Transferase (GST) from Schistosoma japonicum


      Affinity

      0.26nM


      Bead properties

      Bead size: ~ 90 μm 

      Storage buffer: 20% EtOH


      Binding capacity
      High binding capacity,10 μL slurry bind about 20 μg of recombinant GST


      Elution
      - SDS sample buffer
      - 0.2 M glycine pH 2.5
      Instead of elution, we recommend on-bead assays like on-bead digestion for MS analysis.


      Stability and Storage

      Shipped at ambient temperature. Upon receipt store at +4°C. Stable for 1 year. Do not freeze.


      Benefits

      • Effective pull-down of GST fusion proteins, low background

      • Significantly higher sensitivity than glutathione cellulose resin and other affinity resins

      • NO heavy & light chain contamination

      • High binding affinity

      • Harsh washing conditions



      Contact

      If you want to sell VHH on our platform, please contact us !

      Phone

      13551086942

      Email

      service@nb-biolab.com

      聯系

      企業郵箱:service@nb-biolab.com


      公司地址:成都市溫江區青啤大道319號


      上班時間:周一至周五


      工作時間:9:00-18:00


      咨詢

      咨詢電話:400-166-9953


      官方微信

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