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      您的位置: 首頁>產品中心>Phage display system>pComb3Xss
      Nano-secondary antibody
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      pComb3Xss

      Category: Phage display system

      pComb3X is the newest of the pComb vectors. Improvements over pComb3 include increased stability and introduction of an asymmetric SfiI cassette for directional cloning of full Fab, scFv, peptide and other protein for phage display. 6xHis and HA tags allow for purification and detection. An amber stop codon was introduced to turn-off expression of the pIII fusion protein by switching to a non-supressor strain of E. coli allowing production of soluble protein without subcloning. Alternatively, the gene for phage protein pIII can be removed by SpeI/NheI digest. pComb3XSS is recommended for preparation of vector for library cloning. The “SS” refers to the double stuffer, a 1200bp stuffer in the Fab light chain cloning region bounded by SacI and XbaI restriction sites and a 300bp stuffer in Fab heavy chain cloning region bound by XhoI and SpeI restriction sites. Also, the 1600bp double stuffer (both stuffers plus the leader sequence between the Fab light chain and heavy chain cloning regions) can be removed by SfiI digest so that non-Fab genes of interest can be cloned. Also available on Addgene: pComb3XTT and pComb3XLambda are only needed at templates for the construction of chimeric Fab libraries as described in Phage Display: A Laboratory Manual. pComb3XTT can also be used as an Fab expression control.

      Product overview Documents References FAQ

      Summy

      Bacterial Resistance(s): Ampicillin

      Growth Temperature: 37°C

      Growth Strain(s): TG1

      Copy number: High Copy

      Insert Size (bp): 1600

      Promoter:lacZ

      Tags / Fusion Proteins

      6x His (C terminal on backbone)

      HA tag (C terminal on backbone)

      geneIII (C terminal on backbone)

      5′ sequencing primer

      5'-AAG ACA GCT ATC GCG ATT GCA G-3'

      3′ sequencing primer

      5'-GCC CCC TTA TTA GCG TTT GCC ATC-3'

      pcomb3xSS.png

      Vector sequence


              1 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg

             61 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta

            121 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg

            181 aattgtgagc ggataacaat tgaattcagg aggaatttaa aatgaaaaag acagctatcg

            241 cgattgcagt ggcactggct ggtttcgcta ccgtggccca ggcggccgag ctcgccatgg

            301 ctggttgggc agcgagtaat aacaatccag cggctgccgt aggcaatagg tatttcatta

            361 tgactgtctc cttggcgact agctagttta gaattcgtaa tcatggtcat agctgtttcc

            421 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg

            481 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc

            541 cgctttccag tcgggaaacc tgtcgtgtta ctaatgatgg tgatggtgat ggctagtttt

            601 gtcacaagat ttgggctcaa ctttcttgtc caccttggtg ttgctgggct tgtgattcac

            661 gttgcagatg taggtctggg tgcccaagct gctggagggc acggtcacca cgctgctgag

            721 ggagtagagt cctgaggact gtaggacagc cgggaaggtg tgcacgccgc tggtcagggc

            781 gcctgagttc cacgacaccg tcgccggttc ggggaagtag tccttgacca ggcagcccag

            841 ggccgctgtg cccccagagg tgctcttgga ggagggtgcc agggggaaga ccgatgggcc

            901 cttggtggag gctgcggaga cggtgaccgt ggtaccagca gaaacctggc caggctccca

            961 ggctcctcat ctatggtaca tccagcaggg ccactggcat cccagacagg ttcagtggca

           1021 gtgggtctgg gacagacttc actctcacca tcagcagact ggagcctgaa gattttgcag

           1081 tgtactactg tcagcagtat ggtggctcac cgtggttcgg ccaagggacc aaggtggaac

           1141 tcaaacgaac tgtggctgca ccatctgtct tcatcttccc gccatctgat gagcagttga

           1201 aatctggaac tgcctctgtt gtgtgcctgc tgaataactt ctatcccaga gaggccaaag

           1261 tacagtggaa ggtggataac gccctccaat cgggtaactc ccaggagagt gtcacagagc

           1321 aggacagcaa ggacagcacc tacagcctca gcagcaccct gacgctgagc aaagcagact

           1381 acgagaaaca caaagtctac gcctgcgaag tcacccatca gggcctgagc ttgcccgtca

           1441 caaagagctt caacagggga gagtgttagt tctagataat taattaggag gaatttaaaa

           1501 tgaaatacct attgcctacg gcagccgctg gattgttatt actcgctgcc caaccagcca

           1561 tggccgaggt gcagctgctc gagatgagcg ataaaattat tcacctgact gacgacagtt

           1621 ttgacacgga tgtactcaaa gcggacgggg cgatcctcgt cgatttctgg gcagagtggt

           1681 gcggtccgtg caaaatgatc gccccgattc tggatgaaat cgctgacgaa tatcagggca

           1741 aactgaccgt tgcaaaactg aacatcgatc aaaaccctgg cactgcgccg aaatatggca

           1801 tccgtggtat cccgactctg ctgctgttca aaaacggtga agtggcggca accaaagtgg

           1861 gtgcactgtc taaaggtcag ttgaaagagt tcctcgacgc taacctggcg tacccgtacg

           1921 acgttccgga ctacggttct actagtggcc aggccggcca gcaccatcac catcaccatg

           1981 gcgcataccc gtacgacgtt ccggactacg cttcttagga gggtggtggc tctgagggtg

           2041 gcggttctga gggtggcggc tctgagggag gcggttccgg tggtggctct ggttccggtg

           2101 attttgatta tgaaaagatg gcaaacgcta ataagggggc tatgaccgaa aatgccgatg

           2161 aaaacgtgct acagtctgac gctaaaggca aacttgattc tgtcgctact gattacggtg

           2221 ctgctatcga tggtttcatt ggtgacgttt ccggccttgc taatggtaat ggtgctactg

           2281 gtgattttgc tggctctaat tcccaaatgg ctcaagtcgg tgacggtgat aattcacctt

           2341 taatgaataa tttccgtcaa tatttacctt ccctccctca atcggttgaa tgtcgccctt

           2401 ttgtctttgg cgctggtaaa ccatatgaat tttctattga ttgtgacaaa ataaacttat

           2461 tccgtggtgt ctttgcgttt cttttatatg ttgccacctt tatgtatgta ttttctacgt

           2521 ttgctaacat actgcgtaat aaggagtctt aagctagcta attaatttaa gcggccgcag

           2581 atctgctctc tgaggaggat ctgggaaatt gtaagcgtta atattttgtt aaaattcgcg

           2641 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct

           2701 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt

           2761 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat

           2821 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca

           2881 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac

           2941 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta

           3001 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg

           3061 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata

           3121 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga

           3181 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca

           3241 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat

           3301 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag

           3361 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc

           3421 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct

           3481 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca

           3541 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt

           3601 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat

           3661 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt

           3721 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta

           3781 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga

           3841 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt

           3901 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc

           3961 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct

           4021 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata

           4081 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt

           4141 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc

           4201 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg

           4261 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact

           4321 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg

           4381 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg

           4441 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac

           4501 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca

           4561 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga

           4621 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc

           4681 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct

           4741 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg

           4801 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct

           4861 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc

           4921 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc

           4981 gaggaagcgg a

      //


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