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      您的位置: 首頁>產品中心>Phage display system>pComb3XTT
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      pComb3XTT

      Category: Phage display system

      pComb3X is the newest of the pComb vectors. Improvements over pComb3 include increased stability and introduction of an asymmetric SfiI cassette for directional cloning of full Fab, scFv, peptide and other protein for phage display. 6xHis and HA tags allow for purification and detection. An amber stop codon was introduced to turn-off expression of the pIII fusion protein by switching to a non-supressor strain of E. coli allowing production of soluble protein without subcloning. Alternatively, the gene for phage protein pIII can be removed by SpeI/NheI digest. The “TT” refers to the human Fab to tetanus toxin which can be used as a template to amplify the kappa light chain constant region and Fd for construction of chimeric antibody libraries .

      Product overview Documents References FAQ

      Summy

      Bacterial Resistance(s): Ampicillin

      • Growth Temperature: 37°C

      • Growth Strain(s): TG1

      • Copy number: High Copy

      • Insert Size (bp): 1600

      • Promoter:lacZ

      Tags / Fusion Proteins


      6x His (C terminal on backbone)

      HA tag (C terminal on backbone)

      geneIII (C terminal on backbone)

      • 5′ sequencing primer

        5'-AAG ACA GCT ATC GCG ATT GCA G-3'

      • 3′ sequencing primer

        5'-GCC CCC TTA TTA GCG TTT GCC ATC-3'

      pcomb3XTT Map.png

      Vector sequence

              1 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg

             61 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta

            121 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg

            181 aattgtgagc ggataacaat tgaattcagg aggaatttaa aatgaaaaag acagctatcg

            241 cgattgcagt ggcactggct ggtttcgcta ccgtggccca ggcggccgag ctcacgcagt

            301 ctccaggcac cctgtctttg tctccagggg aaagagccac cctctcctgc agggccagtc

            361 acagtgttag cagggcctac ttagcctggt accagcagaa acctggccag gctcccaggc

            421 tcctcatcta tggtacatcc agcagggcca ctggcatccc agacaggttc agtggcagtg

            481 ggtctgggac agacttcact ctcaccatca gcagactgga gcctgaagat tttgcagtgt

            541 actactgtca gcagtatggt ggctcaccgt ggttcggcca agggaccaag gtggaactca

            601 aacgaactgt ggctgcacca tctgtcttca tcttcccgcc atctgatgag cagttgaaat

            661 ctggaactgc ctctgttgtg tgcctgctga ataacttcta tcccagagag gccaaagtac

            721 agtggaaggt ggataacgcc ctccaatcgg gtaactccca ggagagtgtc acagagcagg

            781 acagcaagga cagcacctac agcctcagca gcaccctgac gctgagcaaa gcagactacg

            841 agaaacacaa agtctacgcc tgcgaagtca cccatcaggg cctgagcttg cccgtcacaa

            901 agagcttcaa caggggagag tgttagttct agataattaa ttaggaggaa tttaaaatga

            961 aatacctatt gcctacggca gccgctggat tgttattact cgctgcccaa ccagccatgg

           1021 ccgaggtgca gctgctcgag cagtctgggg ctgaggtgaa gaagcctggg tcctcggtga

           1081 aggtctcctg cagggcttct ggaggcacct tcaacaatta tgccatcagc tgggtgcgac

           1141 aggcccctgg acaagggctt gagtggatgg gagggatctt ccctttccgt aatacagcaa

           1201 agtacgcaca acacttccag ggcagagtca ccattaccgc ggacgaatcc acgggcacag

           1261 cctacatgga gctgagcagc ctgagatctg aggacacggc catatattat tgtgcgagag

           1321 gggatacgat ttttggagtg accatgggat actacgctat ggacgtctgg ggccaaggga

           1381 ccacggtcac cgtctccgca gcctccacca agggcccatc ggtcttcccc ctggcaccct

           1441 cctccaagag cacctctggg ggcacagcgg ccctgggctg cctggtcaag gactacttcc

           1501 ccgaaccggt gacggtgtcg tggaactcag gcgccctgac cagcggcgtg cacaccttcc

           1561 cggctgtcct acagtcctca ggactctact ccctcagcag cgtggtgacc gtgccctcca

           1621 gcagcttggg cacccagacc tacatctgca acgtgaatca caagcccagc aacaccaagg

           1681 tggacaagaa agttgagccc aaatcttgtg acaaaactag tggccaggcc ggccagcacc

           1741 atcaccatca ccatggcgca tacccgtacg acgttccgga ctacgcttct taggagggtg

           1801 gtggctctga gggtggcggt tctgagggtg gcggctctga gggaggcggt tccggtggtg

           1861 gctctggttc cggtgatttt gattatgaaa agatggcaaa cgctaataag ggggctatga

           1921 ccgaaaatgc cgatgaaaac gtgctacagt ctgacgctaa aggcaaactt gattctgtcg

           1981 ctactgatta cggtgctgct atcgatggtt tcattggtga cgtttccggc cttgctaatg

           2041 gtaatggtgc tactggtgat tttgctggct ctaattccca aatggctcaa gtcggtgacg

           2101 gtgataattc acctttaatg aataatttcc gtcaatattt accttccctc cctcaatcgg

           2161 ttgaatgtcg cccttttgtc tttggcgctg gtaaaccata tgaattttct attgattgtg

           2221 acaaaataaa cttattccgt ggtgtctttg cgtttctttt atatgttgcc acctttatgt

           2281 atgtattttc tacgtttgct aacatactgc gtaataagga gtcttaagct agctaattaa

           2341 tttaagcggc cgcagatctg ctctctgagg aggatctggg aaattgtaag cgttaatatt

           2401 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa

           2461 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca

           2521 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc

           2581 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg

           2641 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg

           2701 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg

           2761 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg

           2821 ccgctacagg gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt

           2881 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg

           2941 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt

           3001 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta

           3061 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc

           3121 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa

           3181 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc

           3241 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt

           3301 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact

           3361 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac

           3421 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata

           3481 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta

           3541 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg

           3601 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat

           3661 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt

           3721 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga

           3781 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa

           3841 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag

           3901 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac

           3961 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc

           4021 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat

           4081 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat

           4141 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct

           4201 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt

           4261 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg

           4321 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta

           4381 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg

           4441 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg

           4501 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc

           4561 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg

           4621 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat

           4681 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc

           4741 agcgagtcag tgagcgagga agcgga

      //



      Barbas, C. F., III; Burton, D. R.; Scott, J.K., Silverman, G.J. Eds. (2001) Phage Display: A Laboratory Manual; Cold Spring Harbor Laboratory Press: Cold Spring Harbor, New York

      Cat / Size / Price

      P002/10ug/¥3000.0

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      If you want to sell VHH on our platform, please contact us !

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      13551086942

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      service@nb-biolab.com

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      企業郵箱:service@nb-biolab.com


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