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      您的位置: 首頁>產品中心>Phage display system>pComb3XLambda
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      pComb3XLambda

      Category: Phage display system

      pComb3X is the newest of the pComb vectors. Improvements over pComb3 include increased stability and introduction of an asymmetric SfiI cassette for directional cloning of full Fab, scFv, peptide and other protein for phage display. 6xHis and HA tags allow for purification and detection. An amber stop codon was introduced to turn-off expression of the pIII fusion protein by switching to a non-supressor strain of E. coli allowing production of soluble protein without subcloning. Alternatively, the gene for phage protein pIII can be removed by SpeI/NheI digest. pComb3XLambda can be used as a template to amplify the lambda light chain constant region for construction of chimeric antibody libraries as described in:

      Barbas, C. F., III; Burton, D. R.; Scott, J.K., Silverman, G.J. Eds. (2001) Phage Display: A Laboratory Manual; Cold Spring Harbor Laboratory Press: Cold Spring Harbor, New York

      Please note that there is a A246V mutation in heavy chain region; however, this vector is meant to be used as a PCR template for the light chain region from base# 613-1016.


      Product overview Documents References FAQ

      summary

      Bacterial Resistance(s): Ampicillin

      • Growth Temperature: 37°C

      • Growth Strain(s): TG1

      • Copy number: High Copy

      • Insert Size (bp): 1600

      • Promoter:lacZ

      • Tags / Fusion Proteins

      • HA tag (C terminal on backbone)

      • 6x His (C terminal on backbone)

      geneIII (C terminal on backbone)

      • 5′ sequencing primer

        5'-AAG ACA GCT ATC GCG ATT GCA G-3'

      • 3′ sequencing primer

        5'-GCC CCC TTA TTA GCG TTT GCC ATC-3'

      pComb3XLambda.png

      Vector sequence

              1 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg

             61 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta

            121 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg

            181 aattgtgagc ggataacaat tgaattcagg aggaatttaa aatgaaaaag acagctatcg

            241 cgattgcagt ggcactggct ggtttcgcta ccgtggccca ggcggccgag ctcgtgctga

            301 ctaacgccct cagtgtctgg ggccccaggg cagagggtca ccatctcctg cactgggagc

            361 agctccaacc tcggggcagc ttatgatgta cactggtacc accaggttcc aggaacggcc

            421 cccaaactcc tcctctatga taacatcaag cggccctcag gggtccctga ccgattctct

            481 ggctccaagt ctggcacctc agcctccctg gccatcactg ggctccaggc tgaggacgag

            541 gctgattatt actgcaacag agtaacagct ggcctcacac gttcggcgga gggaccaagc

            601 tgaccgtcct aagtcagccc aaggctgccc cctcggtcac tctgttcccg ccctcctctg

            661 aggagcttca agccaacaag gccacactgg tgtgtctcat aagtgacttc tacccgggag

            721 ccgtgacagt ggcctggaag gcagatggca gccccgtcaa ggcgggagtg gagaccacca

            781 caccctccaa acaaagcaac aacaagtacg cggccagcag ctatctgagc ctgacgcctg

            841 agcagtggaa gtcccacaga agctacagct gccaggtcac gcatgaaggg agcaccgtgg

            901 agaagacagt ggcccctaca gaatgttcat aattctagat aattaattag gaggaattta

            961 aaatgaaata cctattgcct acggcagccg ctggattgtt attactcgct gcccaaccag

           1021 ccatggccga ggtgcagctg ctcgagcagt ctggggctga ggtgaagaag cctgggtcct

           1081 cggtgaaggt ctcctgcagg gcttctggag gcaccttcaa caattatgcc atcagctggg

           1141 tgcgacaggc ccctggacaa gggcttgagt ggatgggagg gatcttccct ttccgtaata

           1201 cagcaaagta cgcacaacac ttccagggca gagtcaccat taccgcggac gaatccacgg

           1261 gcacagccta catggagctg agcagcctga gatctgagga cacggccata tattattgtg

           1321 cgagagggga tacgattttt ggagtgacca tgggatacta cgctatggac gtctggggcc

           1381 aagggaccac ggtcaccgtc tccgcagcct ccaccaaggg cccatcggtc ttccccctgg

           1441 caccctcctc caagagcacc tctgggggca cagcggccct gggctgcctg gtcaaggact

           1501 acttccccga accggtgacg gtgtcgtgga actcaggcgc cctgaccagc ggcgtgcaca

           1561 ccttcccggc tgtcctacag tcctcaggac tctactccct cagcagcgtg gtgaccgtgc

           1621 cctccagcag cttgggcacc cagacctaca tctgcaacgt gaatcacaag cccagcaaca

           1681 ccaaggtgga caagaaagtt gagcccaaat cttgtgacaa aactagtggc caggccggcc

           1741 agcaccatca ccatcaccat ggcgcatacc cgtacgacgt tccggactac gcttcttagg

           1801 agggtggtgg ctctgagggt ggcggttctg agggtggcgg ctctgaggga ggcggttccg

           1861 gtggtggctc tggttccggt gattttgatt atgaaaagat ggcaaacgct aataaggggg

           1921 ctatgaccga aaatgccgat gaaaacgtgc tacagtctga cgctaaaggc aaacttgatt

           1981 ctgtcgctac tgattacggt gctgctatcg atggtttcat tggtgacgtt tccggccttg

           2041 ctaatggtaa tggtgctact ggtgattttg ctggctctaa ttcccaaatg gctcaagtcg

           2101 gtgacggtga taattcacct ttaatgaata atttccgtca atatttacct tccctccctc

           2161 aatcggttga atgtcgccct tttgtctttg gcgctggtaa accatatgaa ttttctattg

           2221 attgtgacaa aataaactta ttccgtggtg tctttgcgtt tcttttatat gttgccacct

           2281 ttatgtatgt attttctacg tttgctaaca tactgcgtaa taaggagtct taagctagct

           2341 aattaattta agcggccgca gatctgctct ctgaggagga tctgggaaat tgtaagcgtt

           2401 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag

           2461 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt

           2521 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga

           2581 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg

           2641 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct

           2701 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc

           2761 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt

           2821 aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct

           2881 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga

           2941 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc

           3001 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg

           3061 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc

           3121 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact

           3181 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc

           3241 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag

           3301 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat

           3361 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt

           3421 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa

           3481 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc

           3541 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg

           3601 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt

           3661 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca

           3721 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat

           3781 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca

           3841 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg

           3901 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg

           3961 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt

           4021 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg

           4081 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata

           4141 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca

           4201 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag

           4261 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc

           4321 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga

           4381 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg

           4441 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac

           4501 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg

           4561 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg

           4621 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct

           4681 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc

           4741 gagcgcagcg agtcagtgag cgaggaagcg ga

      //



      Barbas, C. F., III; Burton, D. R.; Scott, J.K., Silverman, G.J. Eds. (2001) Phage Display: A Laboratory Manual; Cold Spring Harbor Laboratory Press: Cold Spring Harbor, New York

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