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      您的位置: 首頁>產品中心>Phage display system>pComb8
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      pComb8

      Category: Phage display system

      pComb8 is nearly identical to the original pComb3 vector but contains the phage pVIII fusion protein for multi-valent expression along the sides of the phage via fusion with pVIII. It was designed for phage display of Fabs which are cloned into two separate cloning cassettes one chain at a time using SacI/XbaI restriction sites (for the light chain) and XhoI/SpeI restriction sites (for the heavy chain). The heavy chain is expressed fused to the pVIII. Both cassettes have the pelB leader sequence, the light chain and heavy chain-geneVIII fusion product assemble in the periplasm to form the Fab fragment. Soluble Fab can be expressed by removing the gene for the pVIII phage fusion protein by SpeI/NheI digest. Sufficient soluble protein is also found in the periplasmic space as a result of proteolysis.

      Product overview Documents References FAQ

      summary

      Bacterial Resistance(s): Ampicillin

      • Growth Temperature: 37°C

      • Growth Strain(s): TG1

      • Copy number: High Copy

      • Insert Size (bp): 1600

      • Promoter:lacZ

      • Tags / Fusion Proteins

      • geneVIII (C terminal on backbone)

      • pcomb8 Map.png


      Assembly of combinatorial antibody libraries on phage surfaces: the gene III site. Barbas CF 3rd, Kang AS, Lerner RA, Benkovic SJ. Proc Natl Acad Sci U S A. 1991 Sep 15;88(18):7978-82. PubMed 1896445

      Cat / Size / Price

      P004/1mL/¥5000.0

      Contact

      If you want to sell VHH on our platform, please contact us !

      Phone

      13551086942

      Email

      service@nb-biolab.com

      聯系

      企業郵箱:service@nb-biolab.com


      公司地址:成都市溫江區青啤大道319號


      上班時間:周一至周五


      工作時間:9:00-18:00


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      咨詢電話:400-166-9953


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