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      您的位置: 首頁>產品中心>Phage display system>pCANTAB5E
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      pCANTAB5E

      Category: Phage display system

      This system is used for antibody gene expression and preparation of recombinant antibody. The host strain TG1 was replicated and expressed by plasmid vector pCANTAB5e, and was cultured on 2 *YT medium at 37 C. The vector pCANTAB5e is used to construct recombinant scFv. It has ampicillin resistance and the size is 4522 bp. The auxiliary phage M13K07 is used to rescue the phage particles. It is Kana-resistant and can be replicated by the auxiliary phage and expressed on the phage surface in the form of fusion. There is a sequence encoding Tag tail peptide (E-Tag) behind the scFv gene. There is an amber termination codon behind the tail peptide. It is located between the scFv gene and the cpIII gene. In the inhibitory bacteria TG1, only 20% of the amber codon is effective, so it can be read through the protein translation process to form scFv-cp. In non-inhibitory strains, such as HB2151, the terminator is recognized, and the scFv gene terminates before the cpIII gene in the translation process, forming an independent antibody protein that remains in the cell membrane gap, and leaks into the culture medium after a long period of culture to form soluble expression.

      Product overview Documents References FAQ

      Summary

      Function E.coli Cloning plasmid

      Promoter: Lac/lac promoter

      Plasmid size: 5216bp

      Plasmid tagging: N-g3 signal, C-E tag

      Prokaryotic resistance: ampicillin Amp

      Clone strain: Escherichia coli DH5α

      Culture conditions: 37 C, aerobic, LB

      Vector sequence

              

              1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt

             61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt

            121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat

            181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt

            241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg

            301 ctgaagatca gttgggtgct cgagtgggtt acatcgaact ggatctcaac agcggtaaga

            361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc

            421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac

            481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg

            541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca

            601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg

            661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg

            721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg

            781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag

            841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg

            901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct

            961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac

           1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact

           1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga

           1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt

           1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct

           1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc

           1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc

           1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc

           1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg

           1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt

           1561 cgtgcataca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg

           1621 agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg

           1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt

           1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag

           1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt

           1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta

           1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt

           1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc

           2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca

           2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc

           2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg

           2221 accatgatta cgccaagctt tggagccttt tttttggaga ttttcaacgt gaaaaaatta

           2281 ttattcgcaa ttcctttagt tgttcctttc tatgcggccc agccggccat ggcccaggtg

           2341 aagctgcagc agtcaggacc tggcctggtg gcgccctcac agagcctgtc catcacatgc

           2401 accgtctcag ggttttcatt aaccagctat ggtgtacact gggttcgcca gcctccagga

           2461 aagggtctgg agtggctggg agtaatatgg gctggtggaa gcacaaacta taattcagct

           2521 ctcaaatcca gactgaacat cagcaaggac aactccaaga gccaagtttt cttaaaaatg

           2581 aacagtctcc aaactgatga cacagccatg tactactgtg ccagaaactg gggcagctac

           2641 tggtacttcg atgtctgggg ccaaggccac ggtcaccgtc tcctcagtgg aggcggttca

           2701 ggcggaggtg gcggaggtgg ctctggcggt ggcggatcgg acattgagct cacccagtct

           2761 ccagcaatca tgtctgcatc tccaggggaa aaggtcacca tgacctgcag ggccagctca

           2821 agtataagtt ccagttactt gcactggtac cagcagaagt caggcgcttc ccccaaaccc

           2881 ttgattcata ggacatccaa cctggcttct ggagtcccag ctcgcttcag tggcagtggg

           2941 tctgggacct cttactctct cacaatcagc agcgtggagg ctgaagatga tgcaacttat

           3001 tactgccagc agtggagtgg ttacccattc acgttcggtg ctgggaccaa gctcgagatc

           3061 aaacgggcgg ccgcaggtgc gccggtgccg tatccggatc cgctggaacc gcgtgccgca

           3121 tagactgttg aaagttgttt agcaaaacct catacagaaa attcatttac taacgtctgg

           3181 aaagacgaca aaactttaga tcgttacgct aactatgagg gctgtctgtg gaatgctaca

           3241 ggcgttgtgg tttgtactgg tgacgaaact cagtgttacg gtacatgggt tcctattggg

           3301 cttgctatcc ctgaaaatga gggtggtggc tctgagggtg gcggttctga gggtggcggt

           3361 tctgagggtg gcggtactaa acctcctgag tacggtgata cacctattcc gggctatact

           3421 tatatcaacc ctctcgacgg cacttatccg cctggtactg agcaaaaccc cgctaatcct

           3481 aatccttctc ttgaggagtc tcagcctctt aatactttca tgtttcagaa taataggttc

           3541 cgaaataggc agggtgcatt aactgtttat acgggcactg ttactcaagg cactgacccc

           3601 gttaaaactt attaccagta cactcctgta tcatcaaaag ccatgtatga cgcttactgg

           3661 aacggtaaat tcagagactg cgctttccat tctggcttta atgaggatcc attcgtttgt

           3721 gaatatcaag gccaatcgtc tgacctgcct caacctcctg tcaatgctgg cggcggctct

           3781 ggtggtggtt ctggtggcgg ctctgagggt ggcggctctg agggtggcgg ttctgagggt

           3841 ggcggctctg agggtggcgg ttccggtggc ggctccggtt ccggtgattt tgattatgaa

           3901 aaaatggcaa acgctaataa gggggctatg accgaaaatg ccgatgaaaa cgcgctacag

           3961 tctgacgcta aaggcaaact tgattctgtc gctactgatt acggtgctgc tatcgatggt

           4021 ttcattggtg acgtttccgg ccttgctaat ggtaatggtg ctactggtga ttttgctggc

           4081 tctaattccc aaatggctca agtcggtgac ggtgataatt cacctttaat gaataatttc

           4141 cgtcaatatt taccttcttt gcctcagtcg gttgaatgtc gcccttatgt ctttggcgct

           4201 ggtaaaccat atgaattttc tattgattgt gacaaaataa acttattccg tggtgtcttt

           4261 gcgtttcttt tatatgttgc cacctttatg tatgtatttt cgacgtttgc taacatactg

           4321 cgtaataagg agtcttaata agaattcact ggccgtcgtt ttacaacgtc gtgactggga

           4381 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg

           4441 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga

           4501 atggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata

           4561 aattgtaaac gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt

           4621 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agcccgagat

           4681 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa

           4741 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccaa

           4801 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc

           4861 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc

           4921 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac

           4981 acccgccgcg cttaatgcgc cgctacaggg cgcgtactat ggttgctttg acgggtgcag

           5041 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc

           5101 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac

           5161 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcga

      //



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