• <code id="4mxcw"><nobr id="4mxcw"><track id="4mxcw"></track></nobr></code>
    <pre id="4mxcw"><small id="4mxcw"><input id="4mxcw"></input></small></pre><nav id="4mxcw"><video id="4mxcw"></video></nav>
      <nav id="4mxcw"><video id="4mxcw"></video></nav>

    <th id="4mxcw"></th>
    1. <object id="4mxcw"><nobr id="4mxcw"><sub id="4mxcw"></sub></nobr></object>
    2. <del id="4mxcw"></del>
    3. 在線咨詢
      QQ
      搜索

      您的位置: 首頁>產品中心>Phage display system>E coli TG1
      Nano-secondary antibody
      Nanoselector
      Smartcapture?
      Nano-tag labels
      Smart Booster
      Smart ligands
      Recombinant HcAb
      Recombinant VHH
      Conventional Secondary Antibody
      Recombinant protein
      Phage display system
      Large Na?ve VHH library

      E coli TG1

      Category: Phage display system

      TG1 is derived from E. coli K-12 strain, which is the fastest growing clone of Escherichia coli at present. At 37 ℃ on the plate, 7h can be cloned. The main strain of phage display, but also can be used to construct plasmid, lacIqZ Delta M15 which can be used for the blue white screening experiments; but without nuclease endA1 mutation, high in vivo nucleic acid enzyme content, using plasmid extraction kit to protein solution to removing nuclease in vivo of extraction plasmids. The TG1 strain needs to be cultured in the LB without antibiotics at ℃, and then 20% of the glycerol is used to preserve the bacteria.

      Product overview Documents References FAQ

      Introduction

      Alternative name      TG1 Escherichia coli Strains,TG1,Escherichia coli

      Strains Resistance    None of resistance

      Culture Medium       LB

      Condition                37℃ ,under the aerobic conditions


      Genotype

      [F?tra D36proABlacIqZΔM15]supEthi-1Δ(lac-proAB)Δ(mcrB-hsdSM)5(rKmK)


      Minimal Medium plate  

             1 M MgCl2?6H2O: Dissolve 20.33 g in distilled water to a final volume of 100 ml and autoclave.

             1 M CaCl2?2H2O: Dissolve 14.7 g in distilled water to a final volume of 100 ml and autoclave.

             1 M thiamine hydrochloride: Dissolve 33.73 g in distilled water to a final volume of 100 ml and sterilize using a 0.22 μm filter.

             20% glucose: Dissolve 20 g of D-(+)-glucose (anhydrous) in distilled water to a final volume of 100 ml and sterilize by filtration through a 0.2 μm filter. Do not autoclave.

            In a 500 ml bottle, dissolve 6 g of Na2HPO4 (dibasic), 3 g of KH2PO4(monobasic) and 1 g of NH4Cl in distilled water to a final volume of 500 ml. adjust the pH to 7.4 with NaOH.

             In a separate 1 liter bottle, add distilled water to 15 g of Bacto-agar to a final Volume of 500 ml. Autoclave both bottles simultaneously to sterilize. Cool both bottles to 50-60°C and combine. Add1 ml of 1 M MgCl2?6H2O, 1 ml of 1 M CaCl2?2H2O, 1 ml of 1 M thiamine hydrochloride and 5 ml of 20% glucose. Pour plates immediately.


      Sambrook, J and Russell DW (2001) Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Laboratory, Cold Spring Harbor, NY)

      Cat / Size / Price

      P008/1mL/¥1,000.00

      Contact

      If you want to sell VHH on our platform, please contact us !

      Phone

      13551086942

      Email

      service@nb-biolab.com

      聯系

      企業郵箱:service@nb-biolab.com


      公司地址:成都市溫江區青啤大道319號


      上班時間:周一至周五


      工作時間:9:00-18:00


      咨詢

      咨詢電話:400-166-9953


      官方微信

      項目咨詢

      Copyright © 2021 成都阿帕克生物科技有限公司 免疫服務,酵母表面展示,噬菌體展示,NGS測序,納米抗體表達,納米抗體人源化 蜀ICP備17007098號

    4. <code id="4mxcw"><nobr id="4mxcw"><track id="4mxcw"></track></nobr></code>
      <pre id="4mxcw"><small id="4mxcw"><input id="4mxcw"></input></small></pre><nav id="4mxcw"><video id="4mxcw"></video></nav>
        <nav id="4mxcw"><video id="4mxcw"></video></nav>

      <th id="4mxcw"></th>
      1. <object id="4mxcw"><nobr id="4mxcw"><sub id="4mxcw"></sub></nobr></object>
      2. <del id="4mxcw"></del>