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      E.coli SS320

      Category: Phage display system

      The E. coli strain SS320 is a non-amber suppressor strain (sometimes called MC1061F′) prepared as high transformation efficiency (≥ 1 × 109 cfu/μg) chemically competent cells for phage display library screening. The SS320 strain harbors an episome that contains the F′ pilus that is required for bacteriophage entry to allow amplification of phage libraries. This episome also contains the tetracycline resistance gene, so cells should be grown in the presence of tetracycline to maintain the F′ pilus.   E. coli strain SS320 are suitable for protein expression, general cloning, blue/white screening, M13 phage work and phage display protein expression.

      Product overview Documents References FAQ

      Introduction

      Strains Resistance    Tet

      Culture Medium       LB

      Condition                37℃ ,under the aerobic conditions

      The E. coli strain SS320 is a non-amber suppressor strain (sometimes called MC1061F′) prepared as high transformation efficiency (≥ 1 × 109 cfu/μg) chemically competent cells for phage display library screening. The SS320 strain harbors an episome that contains the F′ pilus that is required for bacteriophage entry to allow amplification of phage libraries. This episome also contains the tetracycline resistance gene, so cells should be grown in the presence of tetracycline to maintain the F′ pilus.   E. coli strain SS320 are suitable for protein expression, general cloning, blue/white screening, M13 phage work and phage display protein expression.


      Genotype

      hsdR mcrB araD139 Δ(araABC-leu)7679ΔlacX74 galUgalK rpsL thi[F′ proAB+lacIqlacZΔM15 Tn10 (tetr)]


      Cat / Size / Price

      P012/1mL/¥1,000.00

      Contact

      If you want to sell VHH on our platform, please contact us !

      Phone

      13551086942

      Email

      service@nb-biolab.com

      聯系

      企業郵箱:service@nb-biolab.com


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