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      您的位置: 首頁>產品中心>Phage display system>E.coli TOP10 F'
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      E.coli TOP10 F'

      Category: Phage display system

      TOP10F′ E. coli are identical to TOP10 cells, with the addition of an F′ episome. The F′ episome carries the tetracycline resistance gene and allows isolation of single-stranded DNA from vectors that have an f1 origin of replication. In addition, the F′ episome carries the lacIq repressor for inducible expression from trc, tac, and lac promoters using IPTG. TOP10F′ cells require IPTG induction for blue/white color screening.

      TOP10F′ cells are lacIq and require IPTG to induce expression from the lac promoter. If blue/white screening is required to select for transformants, spread 40 μl of 40 mg/ml X-Gal in dimethylformamide and 40 μl of 100 mM IPTG on top of the agar. Let the X-Gal and IPTG diffuse into the agar for approximately 1 hour.

      Product overview Documents References FAQ

      Introduction

      Strains Resistance     tetracycline resistance

      Culture Medium       LB

      Condition                37℃ ,under the aerobic conditions


      Genotype

      F'[lacIqTn10(tetR)] mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL endA1nupG 


      Cat / Size / Price

      P016/1mL/¥1,000.00

      Contact

      If you want to sell VHH on our platform, please contact us !

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      13551086942

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      service@nb-biolab.com

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